› 6aw7

X-ray diffraction
2.15Å resolution

2.15A resolution structure of SAH bound catechol O-methyltransferase (COMT) from Nannospalax galili

Released:
DOI: 10.2210/pdb6aw7/pdb
Model geometry
Fit model/data
PDB entry 6aw7 coloured by chain, front view.
PDB entry 6aw7 coloured by chain, side view.
PDB entry 6aw7 coloured by chain, top view.
Source organism: Nannospalax galili
Entry authors: Lovell S, Mehzabeen N, Battaile KP, Deng Y, Hanzlik RP, Shams I, Moskovitz J

Function and Biology Details

Reaction catalysed: 
a catechol + S-adenosyl-L-methionine = a guaiacol + S-adenosyl-L-homocysteine + H(+).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105057 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catechol O-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 25.72 KDa
Source organism: Nannospalax galili
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A452CSQ0 (Residues: 1-228; Coverage: 100%)
Sequence domains: O-methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 61.353Å b: 86.671Å c: 104.116Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.214
Expression system: Escherichia coli BL21(DE3)

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