› 6b09

X-ray diffraction
3.2Å resolution

Function and Biology Details

Reactions catalysed: 
5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] + H(2)O = N(7)-methylguanosine 5'-diphosphate + 5'-phospho-[mRNA]
IDP + H(2)O = IMP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188436 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
U8 snoRNA-decapping enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 195 amino acids
Theoretical weight: 21.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96DE0 (Residues: 1-195; Coverage: 100%)
Gene name: NUDT16
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

4 bound ligands:
Ligand C7G 2 x C7G
Ligand MG 4 x MG
Ligand CL 1 x CL
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ DW
Spacegroup: C2
Unit cell:
a: 113.395Å b: 47.063Å c: 75.792Å
α: 90° β: 108.74° γ: 90°
R-values:
R R work R free
0.257 0.256 0.279
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Recent insights into noncanonical 5' capping and decapping of RNA.
Doamekpor et al. (2022)
1 more

4 mentions without citation

Opening of the human epithelial calcium channel TRPV6.
McGoldrick et al. (2018)
3 more