› 6b44

Electron Microscopy
2.9Å resolution

Cryo-EM structure of Type I-F CRISPR crRNA-guided Csy surveillance complex with bound target dsDNA

Released:
DOI: 10.2210/pdb6b44/pdb
PDB entry 6b44 coloured by chain, front view.
PDB entry 6b44 coloured by chain, side view.
PDB entry 6b44 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Primary publication:
Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex.
Guo TW, Bartesaghi A, Yang H, Falconieri V, Rao P, Merk A, Eng ET, Raczkowski AM, Fox T, Earl LA, Patel DJ, Subramaniam S
Cell 171 414-426.e12 (2017)
PMID: 28985564
Related structures: EMD-7048

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-119597 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (7 distinct):
CRISPR-associated protein Csy1 Chain: A
Molecule details ›
Chain: A
Length: 436 amino acids
Theoretical weight: 49.34 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02ML9 (Residues: 1-434; Coverage: 100%)
Gene names: PA14_33330, csy1
Sequence domains: CRISPR-associated protein (Cas_Csy1)
CRISPR-associated protein Csy2 Chain: B
Molecule details ›
Chain: B
Length: 329 amino acids
Theoretical weight: 36.45 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM0 (Residues: 1-327; Coverage: 100%)
Gene names: PA14_33320, csy2
Sequence domains: CRISPR-associated protein (Cas_Csy2)
CRISPR-associated protein Csy3 Chains: C, D, E, F, G, H
Molecule details ›
Chains: C, D, E, F, G, H
Length: 344 amino acids
Theoretical weight: 37.78 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM1 (Residues: 1-342; Coverage: 100%)
Gene names: PA14_33310, csy1-3, csy3
Sequence domains: CRISPR-associated protein (Cas_Csy3)
CRISPR-associated endonuclease Cas6/Csy4 Chain: L
Molecule details ›
Chain: L
Length: 189 amino acids
Theoretical weight: 21.63 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM2 (Residues: 1-187; Coverage: 100%)
Gene names: PA14_33300, cas6f, csy4
Sequence domains: CRISPR-associated protein (Cas_Csy4)
Structure domains: CRISPR-associated endoribonuclease Cas6/Csy4
Target DNA strand (41-MER) Chain: N
Molecule details ›
Chain: N
Length: 49 nucleotides
Theoretical weight: 15.19 KDa
Non-target DNA strand (5'-D(P*CP*AP*GP*TP*CP*AP*TP*CP*AP*CP*CP*AP*A)-3') Chain: O
Molecule details ›
Chain: O
Length: 27 nucleotides
Theoretical weight: 8.22 KDa
Pseudomonas aeruginosa strain SMC4485 CRISPR repeat sequence Chain: M
Molecule details ›
Chain: M
Length: 60 nucleotides
Theoretical weight: 19.27 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-7048
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

29 review citations

Cryo-EM in drug discovery: achievements, limitations and prospects.
Renaud et al. (2018)
28 more

7 mentions without citation

Mechanisms of Type I-E and I-F CRISPR-Cas Systems in Enterobacteriaceae.
Xue et al. (2019)
6 more