› 6b46

Electron Microscopy
3.1Å resolution

Cryo-EM structure of Type I-F CRISPR crRNA-guided Csy surveillance complex with bound anti-CRISPR protein AcrF1

Released:
DOI: 10.2210/pdb6b46/pdb
PDB entry 6b46 coloured by chain, front view.
PDB entry 6b46 coloured by chain, side view.
PDB entry 6b46 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex.
Guo TW, Bartesaghi A, Yang H, Falconieri V, Rao P, Merk A, Eng ET, Raczkowski AM, Fox T, Earl LA, Patel DJ, Subramaniam S
Cell 171 414-426.e12 (2017)
PMID: 28985564
Related structures: EMD-7050

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-125824 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated protein Csy3 Chains: C, D, E, F, G, H
Molecule details ›
Chains: C, D, E, F, G, H
Length: 344 amino acids
Theoretical weight: 37.78 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM1 (Residues: 1-342; Coverage: 100%)
Gene names: PA14_33310, csy1-3, csy3
Sequence domains: CRISPR-associated protein (Cas_Csy3)
Anti-CRISPR protein AcrF1 Chains: I, J
Molecule details ›
Chains: I, J
Length: 80 amino acids
Theoretical weight: 8.97 KDa
Source organism: Pseudomonas phage JBD30
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: L7P7M1 (Residues: 1-78; Coverage: 100%)
Gene name: JBD30_035
Sequence domains: Anti-CRISPR protein Acr30-35/AcrF1
Structure domains: Viral Envelope Glycoprotein; domain 2
CRISPR-associated endonuclease Cas6/Csy4 Chain: L
Molecule details ›
Chain: L
Length: 189 amino acids
Theoretical weight: 21.63 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM2 (Residues: 1-187; Coverage: 100%)
Gene names: PA14_33300, cas6f, csy4
Sequence domains: CRISPR-associated protein (Cas_Csy4)
Structure domains: CRISPR-associated endoribonuclease Cas6/Csy4
Pseudomonas aeruginosa strain SMC4485 CRISPR repeat sequence Chain: M
Molecule details ›
Chain: M
Length: 60 nucleotides
Theoretical weight: 19.27 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-7050
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

Cryo-EM in drug discovery: achievements, limitations and prospects.
Renaud et al. (2018)
26 more

3 mentions without citation

Keeping crispr in check: diverse mechanisms of phage-encoded anti-crisprs.
Trasanidou et al. (2019)
2 more