› 6b4k

X-ray diffraction
2.2Å resolution

Crystal structure of human DDX19B(AMPPNP)

Released:
DOI: 10.2210/pdb6b4k/pdb
PDB entry 6b4k coloured by chain, front view.
PDB entry 6b4k coloured by chain, side view.
PDB entry 6b4k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional analysis of mRNA export regulation by the nuclear pore complex.
Lin DH, Correia AR, Cai SW, Huber FM, Jette CA, Hoelz A
Nat Commun 9 2319 (2018)
PMID: 29899397

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193966 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase DDX19B Chains: A, B
Molecule details ›
Chains: A, B
Length: 430 amino acids
Theoretical weight: 48.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UMR2 (Residues: 54-479; Coverage: 89%)
Gene names: DBP5, DDX19, DDX19B, TDBP
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ANP 2 x ANP
Ligand MLI 3 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 83.233Å b: 45.364Å c: 127.391Å
α: 90° β: 97.03° γ: 90°
R-values:
R R work R free
0.209 0.207 0.248
Expression system: Escherichia coli

Quick links

Citations

14 review citations

The Structure of the Nuclear Pore Complex (An Update).
Lin et al. (2019)
13 more

2 mentions without citation

Host DDX Helicases as Possible SARS-CoV-2 Proviral Factors: A Structural Overview of Their Hijacking Through Multiple Viral Proteins.
Squeglia et al. (2020)
1 more