› 6b5c

X-ray diffraction
2.4Å resolution

Structural Basis for Katanin Self-Assembly

Released:
DOI: 10.2210/pdb6b5c/pdb
PDB entry 6b5c coloured by chain, front view.
PDB entry 6b5c coloured by chain, side view.
PDB entry 6b5c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for disassembly of katanin heterododecamers.
Nithianantham S, McNally FJ, Al-Bassam J
J Biol Chem 293 10590-10605 (2018)
PMID: 29752405

Function and Biology Details

Reaction catalysed: 
n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189772 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Katanin p60 ATPase-containing subunit A-like 1 Chain: A
Molecule details ›
Chain: A
Length: 307 amino acids
Theoretical weight: 34.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BW62 (Residues: 184-490; Coverage: 63%)
Gene name: KATNAL1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 40.1Å b: 61.19Å c: 117.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.253
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Microtubule-severing enzymes: From cellular functions to molecular mechanism.
McNally et al. (2018)
2 more

1 mention without citation

Structural basis for disassembly of katanin heterododecamers.
Nithianantham et al. (2018)