› 6b63

X-ray diffraction
2.7Å resolution

IMPase (AF2372) with 25 mM Asp

Released:
DOI: 10.2210/pdb6b63/pdb
PDB entry 6b63 coloured by chain, front view.
PDB entry 6b63 coloured by chain, side view.
PDB entry 6b63 coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Entry authors: Goldstein RI, Roberts M

Function and Biology Details

Reactions catalysed: 
D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Myo-inositol phosphate + H(2)O = myo-inositol + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128239 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-1,6-bisphosphatase/inositol-1-monophosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 28 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O30298 (Residues: 1-252; Coverage: 100%)
Gene names: AF_2372, suhB
Sequence domains: Inositol monophosphatase family
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand CU7 4 x CU7
Ligand PO4 2 x PO4
Ligand MG 6 x MG
Ligand SO4 2 x SO4
Ligand EPE 2 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P32
Unit cell:
a: 89.645Å b: 89.645Å c: 101.915Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.172 0.213
Expression system: Escherichia coli

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