› 6b73

X-ray diffraction
3.1Å resolution

Crystal Structure of a nanobody-stabilized active state of the kappa-opioid receptor

Released:
DOI: 10.2210/pdb6b73/pdb
PDB entry 6b73 coloured by chain, front view.
PDB entry 6b73 coloured by chain, side view.
PDB entry 6b73 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the Nanobody-Stabilized Active State of the Kappa Opioid Receptor.
Che T, Majumdar S, Zaidi SA, Ondachi P, McCorvy JD, Wang S, Mosier PD, Uprety R, Vardy E, Krumm BE, Han GW, Lee MY, Pardon E, Steyaert J, Huang XP, Strachan RT, Tribo AR, Pasternak GW, Carroll FI, Stevens RC, Cherezov V, Katritch V, Wacker D, Roth BL
Cell 172 55-67.e15 (2018)
PMID: 29307491

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230827 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kappa-type opioid receptor; Soluble cytochrome b562 Chains: A, B
Molecule details ›
Chains: A, B
Length: 418 amino acids
Theoretical weight: 47.03 KDa
Source organisms: Expression system: unidentified baculovirus
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
  • Canonical: P41145 (Residues: 54-358; Coverage: 80%)
Gene names: OPRK, OPRK1, cybC
Sequence domains:
Structure domains: Rhodopsin 7-helix transmembrane proteins
Nanobody Chains: C, D
Molecule details ›
Chains: C, D
Length: 134 amino acids
Theoretical weight: 14.91 KDa
Source organism: Lama glama
Expression system: Lama glama

Ligands and Environments

3 bound ligands:
Ligand CVV 2 x CVV
Ligand CLR 2 x CLR
Ligand OLA 1 x OLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 62.31Å b: 150.75Å c: 100.28Å
α: 90° β: 105.66° γ: 90°
R-values:
R R work R free
0.255 0.254 0.275
Expression systems:
  • unidentified baculovirus
  • Lama glama

Quick links

Citations

55 review citations

Mechanisms of signalling and biased agonism in G protein-coupled receptors.
Wootten et al. (2018)
54 more

66 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
65 more