› 6b9i

X-ray diffraction
1.93Å resolution

The crystal structure of the Staphylococcus aureus Fatty acid Kinase (Fak) B1 protein loaded with 14-Methylhexadecanoic Acid (Anteiso C17:0) to 1.93 Angstrom resolution

Released:
DOI: 10.2210/pdb6b9i/pdb
PDB entry 6b9i coloured by chain, front view.
PDB entry 6b9i coloured by chain, side view.
PDB entry 6b9i coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Acyl-chain selectivity and physiological roles of Staphylococcus aureus fatty acid-binding proteins.
Cuypers MG, Subramanian C, Gullett JM, Frank MW, White SW, Rock CO
J Biol Chem 294 38-49 (2019)
PMID: 30429218

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197862 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DegV domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 288 amino acids
Theoretical weight: 32.1 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: X5EH37 (Residues: 1-288; Coverage: 100%)
Gene names: CV021_09110, EP54_02745, EQ90_03735, FAF17_01600, GO814_03395, GO942_03220, GQX37_05295, GZ156_03925, GZ163_04000, HMPREF3211_01094, NCTC13131_00828, NCTC6133_00899, NCTC7878_03402, QU38_10795, SAMEA2078260_01064, SAMEA2078588_00802, SAMEA2080344_01038, SAMEA2081063_00598, SAMEA4008575_00348, SAMEA70146418_00728
Sequence domains: Uncharacterised protein, DegV family COG1307
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand D0G 2 x D0G
Ligand GOL 11 x GOL
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P1
Unit cell:
a: 32.991Å b: 54.17Å c: 83.901Å
α: 104.71° β: 90° γ: 107.73°
R-values:
R R work R free
0.168 0.166 0.206
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Lipid and fatty acid metabolism in trypanosomatids.
Parreira de Aquino et al. (2021)
1 more

1 mention without citation

Acyl-chain selectivity and physiological roles of Staphylococcus aureus fatty acid-binding proteins.
Cuypers et al. (2019)