Function and Biology Details
Reaction catalysed:
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
Talin-1 and Ras-related protein Rap-1b (preferred)
PDBe Complex ID:
PDB-CPX-150643 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Talin-1
Molecule details ›
Chain: A
Length: 93 amino acids
Theoretical weight: 10.61 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: N-terminal or F0 domain of Talin-head FERM
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Length: 93 amino acids
Theoretical weight: 10.61 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P26039 (Residues: 1-86; Coverage: 3%)
Sequence domains: N-terminal or F0 domain of Talin-head FERM
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Ras-related protein Rap-1b
Molecule details ›
Chain: B
Length: 168 amino acids
Theoretical weight: 19.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Length: 168 amino acids
Theoretical weight: 19.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P61224 (Residues: 1-167; Coverage: 91%)
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
wwPDB Validation report is not available for this NMR entry.
Chemical shift assignment:
74%
Refinement method:
simulated annealing
Expression system: Escherichia coli BL21(DE3)
