› 6ba9

X-ray diffraction
1.4Å resolution

YbtT - Type II thioesterase from Yersiniabactin NRPS/PKS biosynthetic pathway- S89A mutant

Released:
DOI: 10.2210/pdb6ba9/pdb
PDB entry 6ba9 coloured by chain, front view.
PDB entry 6ba9 coloured by chain, side view.
PDB entry 6ba9 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
YbtT is a low-specificity type II thioesterase that maintains production of the metallophore yersiniabactin in pathogenic enterobacteria.
Ohlemacher SI, Xu Y, Kober DL, Malik M, Nix JC, Brett TJ, Henderson JP
J Biol Chem 293 19572-19585 (2018)
PMID: 30355735

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100337 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioesterase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 29.4 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A061LQM0 (Residues: 5-267; Coverage: 99%)
Gene names: BvCmsKKP061_00546, C3F40_00565, D3C88_12360, D4M65_13485, D4N09_00915, DIV22_11715, EIA08_14930, EWK56_23660, FPI65_12170, HL601_15330, HVV39_01325, NCTC10865_02596, NCTC8960_04784, NCTC9075_02775, irp4, ybtT
Sequence domains: Thioesterase domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P4212
Unit cell:
a: 82.45Å b: 82.45Å c: 82.043Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.18
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Engineering Siderophore Biosynthesis and Regulation Pathways to Increase Diversity and Availability.
Puja et al. (2023)
5 other articles

2 mentions without citation

YbtT is a low-specificity type II thioesterase that maintains production of the metallophore yersiniabactin in pathogenic enterobacteria.
Ohlemacher et al. (2018)
1 more