› 6bbm

Electron Microscopy
4.1Å resolution

Mechanisms of Opening and Closing of the Bacterial Replicative Helicase: The DnaB Helicase and Lambda P Helicase Loader Complex

Released:
DOI: 10.2210/pdb6bbm/pdb
PDB entry 6bbm coloured by chain, front view.
PDB entry 6bbm coloured by chain, side view.
PDB entry 6bbm coloured by chain, top view.
Source organisms:
Primary publication:
Mechanisms of opening and closing of the bacterial replicative helicase.
Chase J, Catalano A, Noble AJ, Eng ET, Olinares PD, Molloy K, Pakotiprapha D, Samuels M, Chait B, des Georges A, Jeruzalmi D
Elife 7 (2018)
PMID: 30582519
Related structures: EMD-7076

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137200 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Replicative DNA helicase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 471 amino acids
Theoretical weight: 52.45 KDa
Source organism: Escherichia coli O111:NM
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ACB0 (Residues: 1-471; Coverage: 100%)
Gene names: JW4012, b4052, dnaB, groP, grpA
Sequence domains:
Replication protein P Chains: V, W, X, Y, Z
Molecule details ›
Chains: V, W, X, Y, Z
Length: 233 amino acids
Theoretical weight: 23.14 KDa
Source organism: Lambdavirus lambda
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03689 (Residues: 1-107; Coverage: 46%)
Gene name: P
Sequence domains: Replication protein P

Ligands and Environments

1 bound ligand:
Ligand ADP 5 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-7076
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

A structural view of bacterial DNA replication.
Oakley AJ. (2019)
2 more

4 mentions without citation

Mechanisms of opening and closing of the bacterial replicative helicase.
Chase et al. (2018)
3 more