› 6bcn

X-ray diffraction
2.5Å resolution

I-LtrI E184D bound to cognate substrate (pre-cleavage complex)

Released:
DOI: 10.2210/pdb6bcn/pdb
PDB entry 6bcn coloured by chain, front view.
PDB entry 6bcn coloured by chain, side view.
PDB entry 6bcn coloured by chain, top view.
Source organism: Leptographium truncatum
Primary publication:
Active site residue identity regulates cleavage preference of LAGLIDADG homing endonucleases.
McMurrough TA, Brown CM, Zhang K, Hausner G, Junop MS, Gloor GB, Edgell DR
Nucleic Acids Res 46 11990-12007 (2018)
PMID: 30357419

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111777 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homing endonuclease LAGLIDADG domain-containing protein Chains: A, C, G, J
Molecule details ›
Chains: A, C, G, J
Length: 315 amino acids
Theoretical weight: 36 KDa
Source organism: Leptographium truncatum
Expression system: Escherichia coli
UniProt:
  • Canonical: C7SWF3 (Residues: 398-712; Coverage: 44%)
Gene names: HEG fusion, rps3
Sequence domains: LAGLIDADG endonuclease
DNA (26-MER) Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 26 nucleotides
Theoretical weight: 8.04 KDa
Source organism: Leptographium truncatum
Expression system: Not provided
DNA (26-MER) Chains: D, F, I, L
Molecule details ›
Chains: D, F, I, L
Length: 27 nucleotides
Theoretical weight: 8.22 KDa
Source organism: Leptographium truncatum
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 13 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P1
Unit cell:
a: 42.65Å b: 64.02Å c: 168.976Å
α: 89.93° β: 90.19° γ: 93.64°
R-values:
R R work R free
0.239 0.237 0.276
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Genome-Editing Technologies: Concept, Pros, and Cons of Various Genome-Editing Techniques and Bioethical Concerns for Clinical Application.
Khan SH. (2019)
2 more

1 mention without citation

Active site residue identity regulates cleavage preference of LAGLIDADG homing endonucleases.
McMurrough et al. (2018)