› 6bd0

X-ray diffraction
1.45Å resolution

I-OnuI K227Y, D236A bound to cognate substrate (pre-cleavage complex)

Released:
DOI: 10.2210/pdb6bd0/pdb
PDB entry 6bd0 coloured by chain, front view.
PDB entry 6bd0 coloured by chain, side view.
PDB entry 6bd0 coloured by chain, top view.
Source organisms:
Entry authors: Brown C, Zhang K, Laforet M, McMurrough TA, Gloor GB, Edgell DR, Junop M

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119598 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homing endonuclease LAGLIDADG domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 34.52 KDa
Source organism: Ophiostoma novo-ulmi subsp. americana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4VWW5 (Residues: 417-715; Coverage: 42%)
Gene names: HEG fusion, rps3
Sequence domains: LAGLIDADG endonuclease
DNA (25-MER) Chain: B
Molecule details ›
Chain: B
Length: 25 nucleotides
Theoretical weight: 7.47 KDa
Source organism: Ophiostoma novo-ulmi
Expression system: Not provided
DNA (25-MER) Chain: C
Molecule details ›
Chain: C
Length: 25 nucleotides
Theoretical weight: 7.88 KDa
Source organism: Ophiostoma novo-ulmi
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 41.88Å b: 66.74Å c: 159.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.19
Expression systems:
  • Escherichia coli
  • Not provided

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