› 6bda

X-ray diffraction
1.88Å resolution

Wild-type I-OnuI bound to A3G substrate (post-cleavage complex)

Released:
DOI: 10.2210/pdb6bda/pdb
PDB entry 6bda coloured by chain, front view.
PDB entry 6bda coloured by chain, side view.
PDB entry 6bda coloured by chain, top view.
Source organisms:
Entry authors: Brown C, Zhang K, Laforet M, McMurrough T, Gloor GB, Edgell DR, Junop M

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119599 (preferred)
Entry contents:
1 distinct polypeptide molecule
4 distinct DNA molecules
Macromolecules (5 distinct):
Homing endonuclease LAGLIDADG domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 307 amino acids
Theoretical weight: 35.19 KDa
Source organism: Ophiostoma novo-ulmi subsp. americana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4VWW5 (Residues: 413-715; Coverage: 42%)
Gene names: HEG fusion, rps3
Sequence domains: LAGLIDADG endonuclease
Cleaved Cognate DNA strand, +11 sense Chain: B
Molecule details ›
Chain: B
Length: 14 nucleotides
Theoretical weight: 4.16 KDa
Source organism: Ophiostoma novo-ulmi
Expression system: Not provided
Cleaved cognate DNA strand, -11 sense Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.28 KDa
Source organism: Ophiostoma novo-ulmi
Expression system: Not provided
Cleaved cognate DNA strand, -11 antisense Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.68 KDa
Source organism: Ophiostoma novo-ulmi
Expression system: Not provided
Cleaved cognate DNA strand, +11 antisense Chain: E
Molecule details ›
Chain: E
Length: 10 nucleotides
Theoretical weight: 3.14 KDa
Source organism: Ophiostoma novo-ulmi
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 42.69Å b: 68.98Å c: 161.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.233
Expression systems:
  • Escherichia coli
  • Not provided

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