› 6bdb

X-ray diffraction
1.5Å resolution

I-OnuI K227Y, D236A bound to A3G substrate (pre-cleavage complex)

Released:
DOI: 10.2210/pdb6bdb/pdb
PDB entry 6bdb coloured by chain, front view.
PDB entry 6bdb coloured by chain, side view.
PDB entry 6bdb coloured by chain, top view.
Source organisms:
Entry authors: Brown C, Zhang K, McMurrough TA, Laforet M, Gloor GB, Edgell DR, Junop M

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119600 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homing endonuclease LAGLIDADG domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 307 amino acids
Theoretical weight: 35.14 KDa
Source organism: Ophiostoma novo-ulmi subsp. americana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4VWW5 (Residues: 413-715; Coverage: 42%)
Gene names: HEG fusion, rps3
Sequence domains: LAGLIDADG endonuclease
DNA (26-MER) Chain: B
Molecule details ›
Chain: B
Length: 26 nucleotides
Theoretical weight: 7.78 KDa
Source organism: Ophiostoma novo-ulmi
Expression system: Not provided
DNA (26-MER) Chain: C
Molecule details ›
Chain: C
Length: 26 nucleotides
Theoretical weight: 8.2 KDa
Source organism: Ophiostoma novo-ulmi
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 43.37Å b: 67.19Å c: 169.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.206
Expression systems:
  • Escherichia coli
  • Not provided

Quick links