› 6bf7

Electron Microscopy
6.5Å resolution

Cryo-EM structure of human insulin degrading enzyme in complex with FAB H11-E heavy chain, FAB H11-E light chain

Released:
DOI: 10.2210/pdb6bf7/pdb
PDB entry 6bf7 coloured by chain, front view.
PDB entry 6bf7 coloured by chain, side view.
PDB entry 6bf7 coloured by chain, top view.
Source organisms:
Primary publication:
Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme.
Zhang Z, Liang WG, Bailey LJ, Tan YZ, Wei H, Wang A, Farcasanu M, Woods VA, McCord LA, Lee D, Shang W, Deprez-Poulain R, Deprez B, Liu DR, Koide A, Koide S, Kossiakoff AA, Li S, Carragher B, Potter CS, Tang WJ
Elife 7 (2018)
PMID: 29596046
Related structures: EMD-7091

Function and Biology Details

Reaction catalysed: 
Degradation of insulin, glucagon and other polypeptides. No action on proteins.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-143691 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Insulin-degrading enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 966 amino acids
Theoretical weight: 111.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14735 (Residues: 46-1011; Coverage: 95%)
Gene name: IDE
Sequence domains:
Immunoglobulin gamma-1 heavy chain Chains: C, E
Molecule details ›
Chains: C, E
Length: 218 amino acids
Theoretical weight: 23.06 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DOX5 (Residues: 1-49, 50-219; Coverage: 48%)
Sequence domains:
Immunoglobulin kappa light chain Chains: D, F
Molecule details ›
Chains: D, F
Length: 211 amino acids
Theoretical weight: 23.09 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DOX7 (Residues: 1-211; Coverage: 99%)
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.5Å
Relevant EMDB volumes: EMD-7091
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Structural Lessons From the Mutant Proinsulin Syndrome.
Dhayalan et al. (2021)
6 more

1 mention without citation

Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme.
Zhang et al. (2018)