› 6bj9

X-ray diffraction
1.53Å resolution

Crystal structure of Acat2 thiolase from Ascaris suum

Released:
DOI: 10.2210/pdb6bj9/pdb
Model geometry
Fit model/data
PDB entry 6bj9 coloured by chain, front view.
PDB entry 6bj9 coloured by chain, side view.
PDB entry 6bj9 coloured by chain, top view.
Source organism: Ascaris suum
Primary publication:
Structural and Biochemical Studies of Substrate Selectivity in Ascaris suum Thiolases.
Blaisse MR, Fu B, Chang MCY
Biochemistry 57 3155-3166 (2018)
PMID: 29381332

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123294 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl-CoA acetyltransferase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 393 amino acids
Theoretical weight: 41.15 KDa
Source organism: Ascaris suum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F1KYX0 (Residues: 24-413; Coverage: 94%)
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand K 2 x K
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.3.1
Unit cell:
a: 71.574Å b: 88.123Å c: 142.265Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.139 0.16
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

The 3-ketoacyl-CoA thiolase: an engineered enzyme for carbon chain elongation of chemical compounds.
Liu et al. (2020)
2 other articles