› 6bn0

X-ray diffraction
1.95Å resolution

Avirulence protein 4 (Avr4) from Cladosporium fulvum bound to the hexasaccharide of chitin

Released:
DOI: 10.2210/pdb6bn0/pdb
PDB entry 6bn0 coloured by chain, front view.
PDB entry 6bn0 coloured by chain, side view.
PDB entry 6bn0 coloured by chain, top view.
Source organism: Fulvia fulva
Primary publication:
Structure of the Cladosporium fulvum Avr4 effector in complex with (GlcNAc)6 reveals the ligand-binding mechanism and uncouples its intrinsic function from recognition by the Cf-4 resistance protein.
Hurlburt NK, Chen LH, Stergiopoulos I, Fisher AJ
PLoS Pathog 14 e1007263 (2018)
PMID: 30148881

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169408 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Race-specific elicitor A4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 79 amino acids
Theoretical weight: 8.83 KDa
Source organism: Fulvia fulva
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00363 (Residues: 35-113; Coverage: 68%)
Gene name: AVR4
Sequence domains: Chitin binding Peritrophin-A domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 39.864Å b: 41.081Å c: 121.365Å
α: 90° β: 97.87° γ: 90°
R-values:
R R work R free
0.17 0.168 0.214
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Fungal effectors, the double edge sword of phytopathogens.
Pradhan et al. (2021)
3 more

5 mentions without citation

Exploiting Structural Modelling Tools to Explore Host-Translocated Effector Proteins.
Amoozadeh et al. (2021)
4 more