› 6bof

X-ray diffraction
1.4Å resolution

Crystal structure of KRAS A146T-GDP demonstrating open switch 1 conformation

Released:
DOI: 10.2210/pdb6bof/pdb
PDB entry 6bof coloured by chain, front view.
PDB entry 6bof coloured by chain, side view.
PDB entry 6bof coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tissue-Specific Oncogenic Activity of KRASA146T.
Poulin EJ, Bera AK, Lu J, Lin YJ, Strasser SD, Paulo JA, Huang TQ, Morales C, Yan W, Cook J, Nowak JA, Brubaker DK, Joughin BA, Johnson CW, DeStefanis RA, Ghazi PC, Gondi S, Wales TE, Iacob RE, Bogdanova L, Gierut JJ, Li Y, Engen JR, Perez-Mancera PA, Braun BS, Gygi SP, Lauffenburger DA, Westover KD, Haigis KM
Cancer Discov 9 738-755 (2019)
PMID: 30952657

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134027 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase KRas, N-terminally processed Chains: A, B
Molecule details ›
Chains: A, B
Length: 168 amino acids
Theoretical weight: 19.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01116 (Residues: 2-169; Coverage: 89%)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3
Unit cell:
a: 78.024Å b: 78.024Å c: 55.863Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.113 0.112 0.145
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

31 review citations

The Frequency of Ras Mutations in Cancer.
Prior et al. (2020)
30 more

5 mentions without citation

The current understanding of KRAS protein structure and dynamics.
Pantsar T. (2020)
4 more