› 6bqn

Electron Microscopy
3.9Å resolution

Cryo-EM structure of ENaC

Released:
DOI: 10.2210/pdb6bqn/pdb
PDB entry 6bqn coloured by chain, front view.
PDB entry 6bqn coloured by chain, side view.
PDB entry 6bqn coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the human epithelial sodium channel by cryo-electron microscopy.
Noreng S, Bharadwaj A, Posert R, Yoshioka C, Baconguis I
Elife 7 (2018)
PMID: 30251954
Related structures: EMD-7130

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-153347 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Amiloride-sensitive sodium channel subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 489 amino acids
Theoretical weight: 54.04 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P37088 (Residues: 111-543; Coverage: 65%)
Gene names: SCNN1, SCNN1A
Sequence domains: Amiloride-sensitive sodium channel
Amiloride-sensitive sodium channel subunit beta Chain: B
Molecule details ›
Chain: B
Length: 495 amino acids
Theoretical weight: 55.04 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P51168 (Residues: 73-515; Coverage: 69%)
Gene name: SCNN1B
Sequence domains: Amiloride-sensitive sodium channel
Amiloride-sensitive sodium channel subunit gamma Chain: C
Molecule details ›
Chain: C
Length: 503 amino acids
Theoretical weight: 55.83 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P51170 (Residues: 78-524; Coverage: 69%)
Gene name: SCNN1G
Sequence domains: Amiloride-sensitive sodium channel
7B1 fab Chain: D
Molecule details ›
Chain: D
Length: 106 amino acids
Theoretical weight: 9.04 KDa
Source organism: Mus musculus
7B1 fab Chain: E
Molecule details ›
Chain: E
Length: 121 amino acids
Theoretical weight: 10.32 KDa
Source organism: Mus musculus
10D4 fab Chain: F
Molecule details ›
Chain: F
Length: 115 amino acids
Theoretical weight: 9.81 KDa
Source organism: Mus musculus
10D4 fab Chain: G
Molecule details ›
Chain: G
Length: 112 amino acids
Theoretical weight: 9.55 KDa
Source organism: Mus musculus

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-7130
Expression system: Homo sapiens

Quick links

Citations

22 review citations

Taste transduction and channel synapses in taste buds.
Taruno et al. (2021)
21 more

22 mentions without citation

Regulating ENaC's gate.
Kleyman et al. (2020)
21 more