› 6brl

X-ray diffraction
2Å resolution

Crystal structure of a glutamate tRNA ligase from Elizabethkingia meningosepticum CCUG26117 in complex with its amino acid

Released:
DOI: 10.2210/pdb6brl/pdb
PDB entry 6brl coloured by chain, front view.
PDB entry 6brl coloured by chain, side view.
PDB entry 6brl coloured by chain, top view.
Source organism: Elizabethkingia meningoseptica
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103753 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 511 amino acids
Theoretical weight: 58.63 KDa
Source organism: Elizabethkingia meningoseptica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1T3HI54 (Residues: 1-503; Coverage: 100%)
Gene names: BMF97_00245, gltX
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GLU 1 x GLU
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 43.26Å b: 111.89Å c: 130.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.214
Expression system: Escherichia coli BL21(DE3)

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