› 6brp

X-ray diffraction
2.39Å resolution

F-box protein form 2

Released:
DOI: 10.2210/pdb6brp/pdb
PDB entry 6brp coloured by chain, front view.
PDB entry 6brp coloured by chain, side view.
PDB entry 6brp coloured by chain, top view.
Source organisms:
Primary publication:
Structural plasticity of D3-D14 ubiquitin ligase in strigolactone signalling.
Shabek N, Ticchiarelli F, Mao H, Hinds TR, Leyser O, Zheng N
Nature 563 652-656 (2018)
PMID: 30464344

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174446 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
F-box/LRR-repeat MAX2 homolog Chains: B, D
Molecule details ›
Chains: B, D
Length: 688 amino acids
Theoretical weight: 75.83 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: Q5VMP0 (Residues: 1-476, 516-720; Coverage: 95%)
Gene names: D3, LOC_Os06g06050, OSJNBa0085L11.6-1, Os06g0154200
Sequence domains: F-box-like
SKP1-like protein 1A Chains: A, C
Molecule details ›
Chains: A, C
Length: 160 amino acids
Theoretical weight: 17.88 KDa
Source organism: Arabidopsis thaliana
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: Q39255 (Residues: 1-160; Coverage: 100%)
Gene names: ASK1, At1g75950, SKP1, SKP1A, T4O12.17, UIP1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 79.451Å b: 130.471Å c: 94.316Å
α: 90° β: 99.38° γ: 90°
R-values:
R R work R free
0.192 0.192 0.218
Expression system: Baculovirus expression vector pFastBac1-HM

Quick links

Citations

16 review citations

Cullin-RING Ubiquitin Ligase Regulatory Circuits: A Quarter Century Beyond the F-Box Hypothesis.
Harper et al. (2021)
15 more

2 mentions without citation

Structural plasticity of D3-D14 ubiquitin ligase in strigolactone signalling.
Shabek et al. (2018)
1 more