› 6bsn

X-ray diffraction
2.15Å resolution

Structure of proline utilization A (PutA) with proline bound in remote sites

Released:
DOI: 10.2210/pdb6bsn/pdb
Model geometry
Fit model/data
PDB entry 6bsn coloured by chain, front view.
PDB entry 6bsn coloured by chain, side view.
PDB entry 6bsn coloured by chain, top view.
Source organism: Bradyrhizobium diazoefficiens USDA 110
Primary publication:
Structural Basis for the Substrate Inhibition of Proline Utilization A by Proline.
Korasick DA, Pemberton TA, Arentson BW, Becker DF, Tanner JJ
OpenAccess logo Molecules 23 (2017)
PMID: 29295473

Function and Biology Details

Reactions catalysed: 
L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH
L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183542 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional protein PutA Chains: A, B
Molecule details ›
Chains: A, B
Length: 1001 amino acids
Theoretical weight: 107.67 KDa
Source organism: Bradyrhizobium diazoefficiens USDA 110
Expression system: Escherichia coli
UniProt:
  • Canonical: Q89E26 (Residues: 1-999; Coverage: 100%)
Gene name: putA
Sequence domains:

Ligands and Environments


Cofactor: Ligand FDA 2 x FDA
2 bound ligands:
Ligand PRO 9 x PRO
Ligand SO4 14 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: C2
Unit cell:
a: 166.757Å b: 194.21Å c: 108.738Å
α: 90° β: 121.39° γ: 90°
R-values:
R R work R free
0.21 0.209 0.239
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Covalent Modification of the Flavin in Proline Dehydrogenase by Thiazolidine-2-Carboxylate.
Campbell et al. (2020)
6 more

1 mention without citation

Structural Basis for the Substrate Inhibition of Proline Utilization A by Proline.
Korasick et al. (2017)