› 6bym

X-ray diffraction
2.2Å resolution

Crystal structure of the sterol-bound second StART domain of yeast Lam4

Released:
DOI: 10.2210/pdb6bym/pdb
PDB entry 6bym coloured by chain, front view.
PDB entry 6bym coloured by chain, side view.
PDB entry 6bym coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis of sterol binding and transport by a yeast StARkin domain.
Jentsch JA, Kiburu I, Pandey K, Timme M, Ramlall T, Levkau B, Wu J, Eliezer D, Boudker O, Menon AK
J Biol Chem 293 5522-5531 (2018)
PMID: 29463678

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153724 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Membrane-anchored lipid-binding protein LAM4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 203 amino acids
Theoretical weight: 22.76 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P38800 (Residues: 946-1145; Coverage: 15%)
Gene names: LAM4, LTC3, YHR080C
Sequence domains: VAD1 Analog of StAR-related lipid transfer domain

Ligands and Environments

1 bound ligand:
Ligand HC3 2 x HC3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P1
Unit cell:
a: 38.179Å b: 47.301Å c: 55.996Å
α: 111.21° β: 101.63° γ: 100.33°
R-values:
R R work R free
0.169 0.166 0.23
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The Vps13 Family of Lipid Transporters and Its Role at Membrane Contact Sites.
Dziurdzik et al. (2021)
7 more

3 mentions without citation

Aster Proteins Facilitate Nonvesicular Plasma Membrane to ER Cholesterol Transport in Mammalian Cells.
Sandhu et al. (2018)
2 more