› 6bzf

X-ray diffraction
2.29Å resolution

Structure of S. cerevisiae Zip2:Spo16 complex, C2 form

Released:
DOI: 10.2210/pdb6bzf/pdb
PDB entry 6bzf coloured by chain, front view.
PDB entry 6bzf coloured by chain, side view.
PDB entry 6bzf coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The conserved XPF:ERCC1-like Zip2:Spo16 complex controls meiotic crossover formation through structure-specific DNA binding.
Arora K, Corbett KD
Nucleic Acids Res 47 2365-2376 (2019)
PMID: 30566683

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147874 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Sporulation-specific protein 16 Chains: A, E
Molecule details ›
Chains: A, E
Length: 216 amino acids
Theoretical weight: 25.38 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P17122 (Residues: 2-198; Coverage: 100%)
Gene names: SPO16, YHR153C
Sequence domains:
Protein ZIP2 Chain: B
Molecule details ›
Chain: B
Length: 206 amino acids
Theoretical weight: 24.63 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P53061 (Residues: 499-704; Coverage: 29%)
Gene names: NRC704, YGL249W, ZIP2
Sporulation-specific protein 16 Chains: C, G
Molecule details ›
Chains: C, G
Length: 216 amino acids
Theoretical weight: 25.35 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P17122 (Residues: 2-198; Coverage: 100%)
Gene names: SPO16, YHR153C
Sequence domains:
Protein ZIP2 Chain: D
Molecule details ›
Chain: D
Length: 206 amino acids
Theoretical weight: 24.66 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P53061 (Residues: 499-704; Coverage: 29%)
Gene names: NRC704, YGL249W, ZIP2
Protein ZIP2 Chains: F, H
Molecule details ›
Chains: F, H
Length: 206 amino acids
Theoretical weight: 24.6 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P53061 (Residues: 499-704; Coverage: 29%)
Gene names: NRC704, YGL249W, ZIP2

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MLY 13 x MLY
Ligand M0H 4 x M0H

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 169.402Å b: 63.589Å c: 199.043Å
α: 90° β: 90.6° γ: 90°
R-values:
R R work R free
0.198 0.196 0.25
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Crossing and zipping: molecular duties of the ZMM proteins in meiosis.
Pyatnitskaya et al. (2019)
2 more

1 mention without citation

The conserved XPF:ERCC1-like Zip2:Spo16 complex controls meiotic crossover formation through structure-specific DNA binding.
Arora et al. (2019)