› 6c07

X-ray diffraction
1.85Å resolution

Crystal Structure of S-Adenosylmethionine synthetase (MetK/Mat) from Cryptosporidium parvum

Released:
DOI: 10.2210/pdb6c07/pdb
Model geometry
Fit model/data
PDB entry 6c07 coloured by chain, front view.
PDB entry 6c07 coloured by chain, side view.
PDB entry 6c07 coloured by chain, top view.
Source organism: Cryptosporidium parvum Iowa II
Primary publication:
Structure of a critical metabolic enzyme: S-adenosylmethionine synthetase from Cryptosporidium parvum.
Ohren J, Parungao GG, Viola RE
Acta Crystallogr F Struct Biol Commun 75 290-298 (2019)
PMID: 30950830

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176945 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 406 amino acids
Theoretical weight: 44.9 KDa
Source organism: Cryptosporidium parvum Iowa II
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5CYE4 (Residues: 1-406; Coverage: 100%)
Gene name: cgd7_2650
Sequence domains:
Structure domains: GMP Synthetase; Chain A, domain 3

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand MG 7 x MG
Ligand K 1 x K
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-B
Unit cell:
a: 134.39Å b: 62.06Å c: 191.583Å
α: 90° β: 90.65° γ: 90°
R-values:
R R work R free
0.16 0.157 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Structure of a critical metabolic enzyme: S-adenosylmethionine synthetase from Cryptosporidium parvum.
Ohren et al. (2019)