› 6c5f

X-ray diffraction
1.4Å resolution

Human D-Dopachrome tautomerase (D-DT)/ macrophage migration inhibitory factor 2 (MIF2) complexed with the selective inhibitor 4-CPPC

Released:
DOI: 10.2210/pdb6c5f/pdb
PDB entry 6c5f coloured by chain, front view.
PDB entry 6c5f coloured by chain, side view.
PDB entry 6c5f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Plasticity in the C-Terminal Region of Macrophage Migration Inhibitory Factor-2 Is Associated with an Induced Fit Mechanism for a Selective Inhibitor.
Pantouris G, Bucala R, Lolis EJ
Biochemistry 57 3599-3605 (2018)
PMID: 29847104

Function and Biology Details

Reaction catalysed: 
D-dopachrome = 5,6-dihydroxyindole + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151620 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-dopachrome decarboxylase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 117 amino acids
Theoretical weight: 12.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30046 (Residues: 2-118; Coverage: 99%)
Gene name: DDT
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

1 bound ligand:
Ligand 7L9 1 x 7L9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P3
Unit cell:
a: 83.936Å b: 83.936Å c: 40.479Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.12 0.118 0.157
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

MIF but not MIF-2 recruits inflammatory macrophages in an experimental polymicrobial sepsis model.
Tilstam et al. (2021)
6 more

2 mentions without citation

Structural Plasticity in the C-Terminal Region of Macrophage Migration Inhibitory Factor-2 Is Associated with an Induced Fit Mechanism for a Selective Inhibitor.
Pantouris et al. (2018)
1 more