› 6c6m

X-ray diffraction
2.5Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172387 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myosin light chain kinase, smooth muscle, deglutamylated form Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 103 amino acids
Theoretical weight: 11.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15746 (Residues: 405-507; Coverage: 5%)
Gene names: MLCK, MLCK1, MYLK, MYLK1
Sequence domains: Immunoglobulin I-set domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P65
Unit cell:
a: 74.693Å b: 74.693Å c: 131.481Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.226 0.272
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Regulation of Intestinal Barrier Function by Microbial Metabolites.
Ghosh et al. (2021)
19 more

5 mentions without citation

Intracellular MLCK1 diversion reverses barrier loss to restore mucosal homeostasis.
Graham et al. (2019)
4 more