› 6c86

X-ray diffraction
2.15Å resolution

Crystal Structure of Lysyl-tRNA Synthetase from Cryptosporidium parvum complexed with L-Lysylsulfamoyl Adenosine

Released:
DOI: 10.2210/pdb6c86/pdb
Model geometry
Fit model/data
PDB entry 6c86 coloured by chain, front view.
PDB entry 6c86 coloured by chain, side view.
PDB entry 6c86 coloured by chain, top view.
Source organism: Cryptosporidium parvum Iowa II
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-176919 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 535 amino acids
Theoretical weight: 61.46 KDa
Source organism: Cryptosporidium parvum Iowa II
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5CR27 (Residues: 46-559; Coverage: 92%)
Gene name: cgd4_2370
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand KAA 2 x KAA
Ligand EDO 4 x EDO
Ligand NA 1 x NA
Ligand SO4 3 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21212
Unit cell:
a: 117.03Å b: 142.87Å c: 73.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.204
Expression system: Escherichia coli BL21(DE3)

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