› 6c90

X-ray diffraction
2.2Å resolution

Human Mtr4 helicase in complex with ZCCHC8-CTD

Released:
DOI: 10.2210/pdb6c90/pdb
PDB entry 6c90 coloured by chain, front view.
PDB entry 6c90 coloured by chain, side view.
PDB entry 6c90 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for MTR4-ZCCHC8 interactions that stimulate the MTR4 helicase in the nuclear exosome-targeting complex.
Puno MR, Lima CD
Proc Natl Acad Sci U S A 115 E5506-E5515 (2018)
PMID: 29844170

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154595 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Exosome RNA helicase MTR4 Chain: A
Molecule details ›
Chain: A
Length: 734 amino acids
Theoretical weight: 83.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P42285 (Residues: 71-600, 843-1042; Coverage: 70%)
Gene names: DOB1, KIAA0052, MTR4, MTREX, SKIV2L2
Sequence domains:
Zinc finger CCHC domain-containing protein 8 Chain: B
Molecule details ›
Chain: B
Length: 52 amino acids
Theoretical weight: 5.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6NZY4 (Residues: 659-707; Coverage: 7%)
Gene name: ZCCHC8

Ligands and Environments

4 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 2 x MG
Ligand MLI 4 x MLI
Ligand TLA 1 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P61
Unit cell:
a: 147.635Å b: 147.635Å c: 101.589Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.19 0.221
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Regulation of long non-coding RNAs and genome dynamics by the RNA surveillance machinery.
Nair et al. (2020)
4 more

7 mentions without citation

RNA helicases are hubs that orchestrate exosome-dependent 3'-5' decay.
Weick et al. (2021)
6 more