› 6c9m

X-ray diffraction
2.8Å resolution

The Human NatA (Naa10/Naa15) amino-terminal acetyltransferase complex

Released:
DOI: 10.2210/pdb6c9m/pdb
PDB entry 6c9m coloured by chain, front view.
PDB entry 6c9m coloured by chain, side view.
PDB entry 6c9m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of Human NatA and Its Regulation by the Huntingtin Interacting Protein HYPK.
Gottlieb L, Marmorstein R
Structure 26 925-935.e8 (2018)
PMID: 29754825

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + an N-terminal-L-alanyl-[protein] = an N-terminal-N(alpha)-acetyl-L-alanyl-[protein] + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154344 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
N-alpha-acetyltransferase 15, NatA auxiliary subunit Chains: A, C
Molecule details ›
Chains: A, C
Length: 866 amino acids
Theoretical weight: 101.43 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BXJ9 (Residues: 1-866; Coverage: 100%)
Gene names: GA19, NAA15, NARG1, NATH, TBDN100
Sequence domains:
N-alpha-acetyltransferase 10 Chains: B, D
Molecule details ›
Chains: B, D
Length: 236 amino acids
Theoretical weight: 26.52 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41227 (Residues: 1-235; Coverage: 100%)
Gene names: ARD1, ARD1A, NAA10, TE2
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments

2 bound ligands:
Ligand IHP 2 x IHP
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 95.11Å b: 171.83Å c: 178.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.242
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

NAA10 as a New Prognostic Marker for Cancer Progression.
Kim et al. (2020)
1 more

15 mentions without citation

Co-translational, Post-translational, and Non-catalytic Roles of N-Terminal Acetyltransferases.
Aksnes et al. (2019)
14 more