› 6caj

Electron Microscopy
2.8Å resolution

Electron cryo-microscopy of the eukaryotic translation initiation factor 2B from Homo sapiens

Released:
DOI: 10.2210/pdb6caj/pdb
PDB entry 6caj coloured by chain, front view.
PDB entry 6caj coloured by chain, side view.
PDB entry 6caj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the nucleotide exchange factor eIF2B reveals mechanism of memory-enhancing molecule.
Tsai JC, Miller-Vedam LE, Anand AA, Jaishankar P, Nguyen HC, Renslo AR, Frost A, Walter P
Science 359 (2018)
PMID: 29599213
Related structures: EMD-7442

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156021 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Translation initiation factor eIF2B subunit epsilon Chains: A, B
Molecule details ›
Chains: A, B
Length: 721 amino acids
Theoretical weight: 80.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13144 (Residues: 1-721; Coverage: 100%)
Gene names: EIF2B5, EIF2BE
Sequence domains: eIF4-gamma/eIF5/eIF2-epsilon
Translation initiation factor eIF2B subunit gamma Chains: I, J
Molecule details ›
Chains: I, J
Length: 452 amino acids
Theoretical weight: 50.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NR50 (Residues: 1-452; Coverage: 100%)
Gene name: EIF2B3
Sequence domains: Nucleotidyl transferase
Translation initiation factor eIF2B subunit alpha Chains: G, H
Molecule details ›
Chains: G, H
Length: 305 amino acids
Theoretical weight: 33.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14232 (Residues: 1-305; Coverage: 100%)
Gene names: EIF2B1, EIF2BA
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 368 amino acids
Theoretical weight: 41.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49770 (Residues: 2-351; Coverage: 100%)
Gene names: EIF2B2, EIF2BB
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit delta Chains: E, F
Molecule details ›
Chains: E, F
Length: 523 amino acids
Theoretical weight: 57.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UI10 (Residues: 1-523; Coverage: 100%)
Gene names: EIF2B4, EIF2BD
Sequence domains: Initiation factor 2 subunit family

Ligands and Environments

1 bound ligand:
Ligand C7B 1 x C7B
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-7442
Expression system: Escherichia coli

Quick links

Citations

26 review citations

The integrated stress response: From mechanism to disease.
Costa-Mattioli et al. (2020)
25 more

4 mentions without citation

eIF2B conformation and assembly state regulate the integrated stress response.
Schoof et al. (2021)
3 more