› 6chd

X-ray diffraction
2.5Å resolution

Crystal Structure of Human Lysyl-tRNA Synthetase complexed with L-Lysylsulfamoyl Adenosine

Released:
DOI: 10.2210/pdb6chd/pdb
PDB entry 6chd coloured by chain, front view.
PDB entry 6chd coloured by chain, side view.
PDB entry 6chd coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Dranow DM, Abendroth J, Lorimer DD

Function and Biology Details

Reaction catalysed: 
ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172170 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 597 amino acids
Theoretical weight: 68.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15046 (Residues: 1-597; Coverage: 100%)
Gene names: KARS, KARS1, KIAA0070
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand KAA 2 x KAA
Ligand SO4 11 x SO4
Ligand EDO 1 x EDO
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P3221
Unit cell:
a: 149.87Å b: 149.87Å c: 105.23Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.159 0.157 0.206
Expression system: Escherichia coli BL21(DE3)

Quick links