› 6cig

X-ray diffraction
1.65Å resolution

CRYSTAL STRUCTURE ANALYSIS OF SELENOMETHIONINE SUBSTITUTED ISOFLAVONE O-METHYLTRANSFERASE

Released:
DOI: 10.2210/pdb6cig/pdb
PDB entry 6cig coloured by chain, front view.
PDB entry 6cig coloured by chain, side view.
PDB entry 6cig coloured by chain, top view.
Source organism: Medicago sativa
Primary publication:
Structures of two natural product methyltransferases reveal the basis for substrate specificity in plant O-methyltransferases.
Zubieta C, He XZ, Dixon RA, Noel JP
Nat Struct Biol 8 271-9 (2001)
PMID: 11224575

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a 7-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 7-methoxyisoflavone
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127675 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isoflavone-7-O-methyltransferase 8 Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 40.13 KDa
Source organism: Medicago sativa
Expression system: Escherichia coli
UniProt:
  • Canonical: O24529 (Residues: 1-352; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
4 bound ligands:
Ligand GOL 2 x GOL
Ligand T3A 1 x T3A
Ligand SO4 3 x SO4
Ligand PG4 1 x PG4
2 modified residues:
Ligand MSE 10 x MSE
Ligand CSX 1 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 145.81Å b: 50.24Å c: 63.74Å
α: 90° β: 106.76° γ: 90°
R-values:
R R work R free
0.148 0.146 0.174
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Natural products and plant disease resistance.
Dixon RA. (2001)
27 more

2 mentions without citation

Functional Diversification and Structural Origins of Plant Natural Product Methyltransferases.
Lashley et al. (2022)
1 more