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› 6cjn

X-ray diffraction
2.4Å resolution

Crystal Structure of Chalcone Isomerase from Medicago Sativa with the G95T mutation

Released:
DOI: 10.2210/pdb6cjn/pdb
Model geometry
Fit model/data
PDB entry 6cjn coloured by chain, front view.
PDB entry 6cjn coloured by chain, side view.
PDB entry 6cjn coloured by chain, top view.
Source organism: Medicago sativa
Primary publication:
Bifunctional Substrate Activation via an Arginine Residue Drives Catalysis in Chalcone Isomerases
Burke JR, La Clair JJ, Philippe RN, Pabis A, Jez JM, Cortina G, Kaltenbach M, Bowman ME, Woods KB, Nelson AT, Tawfik DS, Kamerlin SCL, Noel JP
ACS Catalysis (2019)

Function and Biology Details

Reaction catalysed: 
a chalcone = a flavanone.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151152 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chalcone--flavanone isomerase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 24.18 KDa
Source organism: Medicago sativa
Expression system: Escherichia coli
UniProt:
  • Canonical: P28012 (Residues: 1-222; Coverage: 100%)
Gene names: CHI-1, CHI1
Sequence domains: Chalcone-flavanone isomerase

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P6522
Unit cell:
a: 90.82Å b: 90.82Å c: 352.423Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.211 0.24
Expression system: Escherichia coli

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