› 6cl5

X-ray diffraction
2.32Å resolution

Structure of P. aeruginosa R1 pyocin fiber PALES_06171 comprising C-terminal residues 323-701

Released:
DOI: 10.2210/pdb6cl5/pdb
Model geometry
Fit model/data
PDB entry 6cl5 coloured by chain, front view.
PDB entry 6cl5 coloured by chain, side view.
PDB entry 6cl5 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa LESB58
Primary publication:
Structure and Analysis of R1 and R2 Pyocin Receptor-Binding Fibers.
Buth SA, Shneider MM, Scholl D, Leiman PG
OpenAccess logo Viruses 10 (2018)
PMID: 30110933

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tail fiber protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 384 amino acids
Theoretical weight: 40.75 KDa
Source organism: Pseudomonas aeruginosa LESB58
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KW03 (Residues: 323-701; Coverage: 54%)
Gene name: HR1
Sequence domains: Putative tail fiber protein gp53-like, C-terminal

Ligands and Environments

3 bound ligands:
Ligand FE 2 x FE
Ligand MG 2 x MG
Ligand NA 6 x NA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 144.504Å b: 155.031Å c: 200.481Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.206
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Reprogramming bacteriophage host range: design principles and strategies for engineering receptor binding proteins.
Dunne et al. (2021)
4 more

3 mentions without citation

Structure and Analysis of R1 and R2 Pyocin Receptor-Binding Fibers.
Buth et al. (2018)
2 more