6cpo

X-ray diffraction
2.4Å resolution

Crystal structure of DR15 presenting the RQ13 peptide

Released:

Function and Biology Details

Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Biochemical function:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135320 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class II histocompatibility antigen, DR alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 182 amino acids
Theoretical weight: 21.19 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01903 (Residues: 26-207; Coverage: 80%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DRB1 beta chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 190 amino acids
Theoretical weight: 22.07 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01911 (Residues: 30-219; Coverage: 80%)
Gene name: HLA-DRB1
Sequence domains:
Structure domains:
Capsid protein p24 Chains: C, F
Molecule details ›
Chains: C, F
Length: 13 amino acids
Theoretical weight: 1.6 KDa
Source organism: HIV-1 M:B_HXB2R
Expression system: Not provided
UniProt:
  • Canonical: P04585 (Residues: 299-311; Coverage: 1%)
Gene name: gag-pol

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 66.74Å b: 73.5Å c: 94.94Å
α: 90° β: 106.95° γ: 90°
R-values:
R R work R free
0.205 0.203 0.246
Expression systems:
  • Homo sapiens
  • Not provided