› 6cqe

X-ray diffraction
1.89Å resolution

Crystal structure of HPK1 kinase domain S171A mutant

Released:
DOI: 10.2210/pdb6cqe/pdb
PDB entry 6cqe coloured by chain, front view.
PDB entry 6cqe coloured by chain, side view.
PDB entry 6cqe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Hematopoietic Progenitor Kinase-1 Structure in a Domain-Swapped Dimer.
Wu P, Sneeringer CJ, Pitts KE, Day ES, Chan BK, Wei B, Lehoux I, Mortara K, Li H, Wu J, Franke Y, Moffat JG, Grogan JL, Heffron TP, Wang W
Structure 27 125-133.e4 (2019)
PMID: 30503777

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187814 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase kinase kinase kinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 295 amino acids
Theoretical weight: 32.98 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q92918 (Residues: 2-293; Coverage: 35%)
Gene names: HPK1, MAP4K1
Sequence domains: Protein kinase domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21212
Unit cell:
a: 76.498Å b: 87.484Å c: 98.991Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.204 0.258
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

A perspective on HPK1 as a novel immuno-oncology drug target.
Sawasdikosol et al. (2020)
10 other articles

1 mention without citation

Multiple conformational states of the HPK1 kinase domain in complex with sunitinib reveal the structural changes accompanying HPK1 trans-regulation.
Johnson et al. (2019)