› 6cro

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF LAMBDA-CRO BOUND TO A CONSENSUS OPERATOR AT 3.0 ANGSTROM RESOLUTION

Released:
DOI: 10.2210/pdb6cro/pdb
PDB entry 6cro coloured by chain, front view.
PDB entry 6cro coloured by chain, side view.
PDB entry 6cro coloured by chain, top view.
Source organism: Lambdavirus lambda
Primary publication:
Crystal structure of lambda-Cro bound to a consensus operator at 3.0 A resolution.
Albright RA, Matthews BW
J Mol Biol 280 137-51 (1998)
PMID: 9653037

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119690 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Regulatory protein cro Chain: A
Molecule details ›
Chain: A
Length: 60 amino acids
Theoretical weight: 6.71 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03040 (Residues: 2-61; Coverage: 91%)
Gene names: cro, lambdap57
Sequence domains: Cro
Structure domains: CRO Repressor
DNA (5'-D(*AP*CP*TP*AP*TP*CP*AP*CP*CP*GP*CP*GP*GP*GP*TP*GP*AP*TP*AP*C)-3') Chain: R
Molecule details ›
Chain: R
Length: 20 nucleotides
Theoretical weight: 6.12 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided
DNA (5'-D(*TP*GP*TP*AP*TP*CP*AP*CP*CP*CP*GP*CP*GP*GP*TP*GP*AP*TP*AP*G)-3') Chain: U
Molecule details ›
Chain: U
Length: 20 nucleotides
Theoretical weight: 6.15 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I213
Unit cell:
a: 102.97Å b: 102.97Å c: 102.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Phosphatidylinositol phosphate kinase: a link between protein kinase and glutathione synthase folds.
Grishin NV. (1999)
2 more

26 mentions without citation

Studying protein-DNA interactions using atomic force microscopy.
Beckwitt et al. (2018)
25 more