› 6cx0

X-ray diffraction
3.5Å resolution

Structure of AtTPC1 D376A

Released:
DOI: 10.2210/pdb6cx0/pdb
Model geometry
Fit model/data
PDB entry 6cx0 coloured by chain, front view.
PDB entry 6cx0 coloured by chain, side view.
PDB entry 6cx0 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for activation of voltage sensor domains in an ion channel TPC1.
Kintzer AF, Green EM, Dominik PK, Bridges M, Armache JP, Deneka D, Kim SS, Hubbell W, Kossiakoff AA, Cheng Y, Stroud RM
Proc Natl Acad Sci U S A 115 E9095-E9104 (2018)
PMID: 30190435

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188240 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Two pore calcium channel protein 1 Chain: A
Molecule details ›
Chain: A
Length: 727 amino acids
Theoretical weight: 84.31 KDa
Source organism: Arabidopsis thaliana
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q94KI8 (Residues: 11-733; Coverage: 99%)
Gene names: At4g03560, CCH1, F9H3.19, FOU2, T5L23.5, TPC1
Sequence domains: Ion transport protein

Ligands and Environments

2 bound ligands:
Ligand CA 5 x CA
Ligand FJ7 1 x FJ7
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 88.47Å b: 154.42Å c: 218.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.32 0.318 0.352
Expression system: Saccharomyces cerevisiae

Quick links

Citations

5 review citations

Membrane mimetic systems in CryoEM: keeping membrane proteins in their native environment.
Autzen et al. (2019)
4 more

3 mentions without citation

Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.
Enkavi et al. (2019)
2 more