› 6czg

X-ray diffraction
2.2Å resolution

Structure of a redesigned beta barrel, b11L5F_LGL

Released:
DOI: 10.2210/pdb6czg/pdb
PDB entry 6czg coloured by chain, front view.
PDB entry 6czg coloured by chain, side view.
PDB entry 6czg coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
De novo design of a fluorescence-activating β-barrel.
Dou J, Vorobieva AA, Sheffler W, Doyle LA, Park H, Bick MJ, Mao B, Foight GW, Lee MY, Gagnon LA, Carter L, Sankaran B, Ovchinnikov S, Marcos E, Huang PS, Vaughan JC, Stoddard BL, Baker D
Nature 561 485-491 (2018)
PMID: 30209393

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
b11L5F_LGL Chains: A, B
Molecule details ›
Chains: A, B
Length: 115 amino acids
Theoretical weight: 12.39 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 84.92Å b: 35.314Å c: 59.554Å
α: 90° β: 90.87° γ: 90°
R-values:
R R work R free
0.231 0.226 0.28
Expression system: Escherichia coli

Quick links

Citations

36 review citations

Advances in protein structure prediction and design.
Kuhlman et al. (2019)
35 more

3 mentions without citation

De novo design of a fluorescence-activating β-barrel.
Dou et al. (2018)
2 more