› 6czp

X-ray diffraction
2.24Å resolution

2.2 Angstrom Resolution Crystal Structure Oxygen-Insensitive NAD(P)H-dependent Nitroreductase NfsB from Vibrio vulnificus in Complex with FMN

Released:
DOI: 10.2210/pdb6czp/pdb
PDB entry 6czp coloured by chain, front view.
PDB entry 6czp coloured by chain, side view.
PDB entry 6czp coloured by chain, top view.
Source organism: Vibrio vulnificus
Entry authors: Minasov G, Wawrzak Z, Skarina T, Grimshaw S, Kwon K, Savchenko A, Satchell KJF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181719 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitroreductase domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 220 amino acids
Theoretical weight: 24.48 KDa
Source organism: Vibrio vulnificus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7MCD2 (Residues: 1-217; Coverage: 100%)
Gene name: VVA1455
Sequence domains: Nitroreductase family
Structure domains: NADH Oxidase

Ligands and Environments

5 bound ligands:
Ligand FMN 8 x FMN
Ligand GOL 3 x GOL
Ligand PEG 3 x PEG
Ligand CL 3 x CL
Ligand PGE 3 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 118.769Å b: 119.659Å c: 131.175Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.177 0.224
Expression system: Escherichia coli BL21(DE3)

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