› 6d1s

X-ray diffraction
3.2Å resolution

Crystal structure of an apo chimeric human alpha1GABAA receptor

Released:
DOI: 10.2210/pdb6d1s/pdb
PDB entry 6d1s coloured by chain, front view.
PDB entry 6d1s coloured by chain, side view.
PDB entry 6d1s coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of neurosteroid anesthetic action on GABAA receptors.
Chen Q, Wells MM, Arjunan P, Tillman TS, Cohen AE, Xu Y, Tang P
Nat Commun 9 3972 (2018)
PMID: 30266951

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-aminobutyric acid receptor subunit alpha-1; Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 324 amino acids
Theoretical weight: 37.23 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: E0SJQ4 (Residues: 22-220; Coverage: 62%)
  • Canonical: P14867 (Residues: 249-340, 415-444; Coverage: 28%)
Gene names: Dda3937_00520, GABRA1
Sequence domains:
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 108.517Å b: 264.846Å c: 109.153Å
α: 90° β: 110.46° γ: 90°
R-values:
R R work R free
0.222 0.22 0.269
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Towards a Comprehensive Understanding of Anesthetic Mechanisms of Action: A Decade of Discovery.
Hemmings et al. (2019)
9 more

2 mentions without citation

Structural basis of neurosteroid anesthetic action on GABAA receptors.
Chen et al. (2018)
1 more