› 6d32

X-ray diffraction
3.75Å resolution

Crystal structure of Xenopus Smoothened in complex with cyclopamine

Released:
DOI: 10.2210/pdb6d32/pdb
PDB entry 6d32 coloured by chain, front view.
PDB entry 6d32 coloured by chain, side view.
PDB entry 6d32 coloured by chain, top view.
Source organism: Xenopus laevis
Primary publication:
Structural Basis of Smoothened Activation in Hedgehog Signaling.
Huang P, Zheng S, Wierbowski BM, Kim Y, Nedelcu D, Aravena L, Liu J, Kruse AC, Salic A
Cell 174 312-324.e16 (2018)
PMID: 29804838

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 612 amino acids
Theoretical weight: 68.98 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0ABE7 (Residues: 15-17, 23-127; Coverage: 94%)
Gene name: cybC
Sequence domains: Cytochrome b562

Ligands and Environments

1 bound ligand:
Ligand CY8 2 x CY8
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 145.778Å b: 79.232Å c: 104.076Å
α: 90° β: 108.51° γ: 90°
R-values:
R R work R free
0.229 0.227 0.26
Expression system: Spodoptera frugiperda

Quick links

Citations

25 review citations

Cellular signalling by primary cilia in development, organ function and disease.
Anvarian et al. (2019)
24 more

10 mentions without citation

Biochemical mechanisms of vertebrate hedgehog signaling.
Kong et al. (2019)
9 more