› 6d3x

X-ray diffraction
1.8Å resolution

Highly Potent and Selective Plasmin Inhibitors Based on the Sunflower Trypsin Inhibitor-1 Scaffold Attenuate Fibrinolysis in Plasma

Released:
DOI: 10.2210/pdb6d3x/pdb
Model geometry
Fit model/data
PDB entry 6d3x coloured by chain, front view.
PDB entry 6d3x coloured by chain, side view.
PDB entry 6d3x coloured by chain, top view.
Source organisms:
Primary publication:
Highly Potent and Selective Plasmin Inhibitors Based on the Sunflower Trypsin Inhibitor-1 Scaffold Attenuate Fibrinolysis in Plasma.
Swedberg JE, Wu G, Mahatmanto T, Durek T, Caradoc-Davies TT, Whisstock JC, Law RHP, Craik DJ
J Med Chem 62 552-560 (2019)
PMID: 30520638

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Lys-|-Xaa > Arg-|-Xaa, higher selectivity than trypsin. Converts fibrin into soluble products.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133234 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Plasmin light chain B Chains: A, B
Molecule details ›
Chains: A, B
Length: 246 amino acids
Theoretical weight: 27.01 KDa
Source organism: Homo sapiens
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: P00747 (Residues: 565-810; Coverage: 31%)
Gene name: PLG
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Trypsin inhibitor 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 14 amino acids
Theoretical weight: 1.61 KDa
Source organism: Helianthus annuus
Expression system: Not provided
UniProt:
  • Canonical: Q4GWU5 (Residues: 40-53; Coverage: 45%)
Gene name: sfti1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 78.728Å b: 80.127Å c: 83.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.216
Expression systems:
  • Komagataella phaffii GS115
  • Not provided

Quick links

Citations

3 review citations

Peptide-based protease inhibitors from plants.
Hellinger et al. (2019)
2 more

2 mentions without citation

Bowman-Birk Inhibitors: Insights into Family of Multifunctional Proteins and Peptides with Potential Therapeutical Applications.
Gitlin-Domagalska et al. (2020)
1 more