› 6d5k

X-ray diffraction
2.85Å resolution

Structure of Human ATP:Cobalamin Adenosyltransferase bound to ATP, and Adenosylcobalamin

Released:
DOI: 10.2210/pdb6d5k/pdb
PDB entry 6d5k coloured by chain, front view.
PDB entry 6d5k coloured by chain, side view.
PDB entry 6d5k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Sacrificial Cobalt-Carbon Bond Homolysis in Coenzyme B12 as a Cofactor Conservation Strategy.
Campanello GC, Ruetz M, Dodge GJ, Gouda H, Gupta A, Twahir UT, Killian MM, Watkins D, Rosenblatt DS, Brunold TC, Warncke K, Smith JL, Banerjee R
J Am Chem Soc 140 13205-13208 (2018)
PMID: 30282455

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-188481 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Corrinoid adenosyltransferase MMAB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 196 amino acids
Theoretical weight: 21.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96EY8 (Residues: 55-250; Coverage: 78%)
Gene name: MMAB
Sequence domains: Cobalamin adenosyltransferase
Structure domains: Cobalamin adenosyltransferase-like

Ligands and Environments

6 bound ligands:
Ligand 5AD 2 x 5AD
Ligand B12 2 x B12
Ligand SO4 6 x SO4
Ligand ATP 1 x ATP
Ligand MG 2 x MG
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P3121
Unit cell:
a: 112.252Å b: 112.252Å c: 117.993Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.192 0.239
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Sharing vitamins: Cobamides unveil microbial interactions.
Sokolovskaya et al. (2020)
11 other articles

3 mentions without citation

Sacrificial Cobalt-Carbon Bond Homolysis in Coenzyme B12 as a Cofactor Conservation Strategy.
Campanello et al. (2018)
2 more