› 6d6v

Electron Microscopy
4.8Å resolution

CryoEM structure of Tetrahymena telomerase with telomeric DNA at 4.8 Angstrom resolution

Released:
DOI: 10.2210/pdb6d6v/pdb
PDB entry 6d6v coloured by chain, front view.
PDB entry 6d6v coloured by chain, side view.
PDB entry 6d6v coloured by chain, top view.
Source organism: Tetrahymena thermophila
Primary publication:
Structure of Telomerase with Telomeric DNA.
Jiang J, Wang Y, SuĊĦac L, Chan H, Basu R, Zhou ZH, Feigon J
Cell 173 1179-1190.e13 (2018)
PMID: 29775593
Related structures: EMD-7821

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-102778 (preferred)
Entry contents:
6 distinct polypeptide molecules
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (8 distinct):
Telomerase reverse transcriptase Chain: A
Molecule details ›
Chain: A
Length: 1117 amino acids
Theoretical weight: 133.49 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: O77448 (Residues: 1-1117; Coverage: 100%)
Gene names: TERT, TTHERM_00112560
Sequence domains:
Telomeric repeat-binding subunit 1 Chain: D
Molecule details ›
Chain: D
Length: 701 amino acids
Theoretical weight: 82.04 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: D2CVN6 (Residues: 1-701; Coverage: 100%)
Gene names: TAP82, TEB1, TTHERM_00218760
Sequence domains: Replication factor-A C terminal domain
Replication protein A 14 kDa subunit Chain: F
Molecule details ›
Chain: F
Length: 121 amino acids
Theoretical weight: 14.01 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: A0A0U8UFF4 (Residues: 1-121; Coverage: 100%)
Gene names: RPA2, TEB3, TTHERM_00439320
Sequence domains: Replication factor A protein 3
Replication protein A 32 kDa subunit Chain: E
Molecule details ›
Chain: E
Length: 269 amino acids
Theoretical weight: 30.99 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: A0A0U8TRG9 (Residues: 1-269; Coverage: 100%)
Gene names: RPA2, TEB2, TTHERM_001113129
Telomerase-associated protein 50 Chain: G
Molecule details ›
Chain: G
Length: 157 amino acids
Theoretical weight: 13.38 KDa
Source organism: Tetrahymena thermophila
La-related protein 7 homolog Chain: H
Molecule details ›
Chain: H
Length: 542 amino acids
Theoretical weight: 64.21 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: W7X6T2 (Residues: 1-542; Coverage: 100%)
Gene names: TAP65, TTHERM_000318539
Sequence domains:
DNA (5'-D(P*GP*TP*TP*GP*GP*GP*GP*TP*TP*GP*GP*GP*GP*TP*TP*GP*GP*GP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 6.06 KDa
RNA (159-MER) Chain: B
Molecule details ›
Chain: B
Length: 159 nucleotides
Theoretical weight: 50.75 KDa
Sequence domains: Ciliate telomerase RNA

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.8Å
Relevant EMDB volumes: EMD-7821

Quick links

Citations

22 review citations

Telomeres and telomerase: three decades of progress.
Shay et al. (2019)
21 more

21 mentions without citation

Structural biology of telomeres and telomerase.
Smith et al. (2020)
20 more