› 6d8z

X-ray diffraction
2.65Å resolution

Crystal Structure of the C-terminal Guanine Nucleotide Exchange Factor Module of Human Trio

Released:
DOI: 10.2210/pdb6d8z/pdb
PDB entry 6d8z coloured by chain, front view.
PDB entry 6d8z coloured by chain, side view.
PDB entry 6d8z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the C-terminal guanine nucleotide exchange factor module of Trio in an autoinhibited conformation reveals its oncogenic potential.
Bandekar SJ, Arang N, Tully ES, Tang BA, Barton BL, Li S, Gutkind JS, Tesmer JJG
Sci Signal 12 (2019)
PMID: 30783010

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131183 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triple functional domain protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 319 amino acids
Theoretical weight: 37.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75962 (Residues: 1960-2275; Coverage: 10%)
Gene name: TRIO
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 59.181Å b: 95.787Å c: 182.431Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.225 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Kalirin and Trio: RhoGEFs in Synaptic Transmission, Plasticity, and Complex Brain Disorders.
Paskus et al. (2020)
4 more

3 mentions without citation

Structure of the C-terminal guanine nucleotide exchange factor module of Trio in an autoinhibited conformation reveals its oncogenic potential.
Bandekar et al. (2019)
2 more