› 6dee

X-ray diffraction
3.04Å resolution

Crystal structure of the C-terminus of Homo sapiens SPIN90 (SH3-protein interacting with Nck), residues 306-722

Released:
DOI: 10.2210/pdb6dee/pdb
PDB entry 6dee coloured by chain, front view.
PDB entry 6dee coloured by chain, side view.
PDB entry 6dee coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the nucleation-promoting factor SPIN90 bound to the actin filament nucleator Arp2/3 complex.
Luan Q, Liu SL, Helgeson LA, Nolen BJ
EMBO J 37 (2018)
PMID: 30322896

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192773 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NCK-interacting protein with SH3 domain Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 46.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NZQ3 (Residues: 306-722; Coverage: 58%)
Gene names: AF3P21, NCKIPSD, SPIN90
Sequence domains: SPIN90/Ldb17, leucine-rich domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 98.504Å b: 98.504Å c: 81.812Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.264 0.259 0.308
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Nucleation, stabilization, and disassembly of branched actin networks.
Gautreau et al. (2022)
16 other articles

2 mentions without citation

Structure of the nucleation-promoting factor SPIN90 bound to the actin filament nucleator Arp2/3 complex.
Luan et al. (2018)
1 more